Journal: Advanced Science

Article Title: Arrayed Magnetic Accelerator Synergistically Enhances Orthodontic Tooth Movement via Microtubule Assembly‐Mediated Periodontal Remodeling

doi: 10.1002/advs.77447

Figure Lengend Snippet: AMOA promotes osteoclast and inflammatory remodeling in periodontal area. (a) Representative images of immunohistochemical staining for CTSK, TNF‐α, IL‐1β. CTSK staining at the periodontal ligament‑alveolar bone interface. Scale bar: 100 µm(CTSK), 20 µm(TNF‐α), and 20 µm(IL‐1β). (b) Statistics of numbers of CTSK positive cells in the alveolar bone and percentage of TNF‐α, IL‐1β positive cells per field in the periodontal ligament of the first molar. Mean ± SD. n = 4. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.001. (c) Diagram of the possible mechanism by which static magnetic field accelerates orthodontic tooth movement.

Article Snippet: Primary antibodies against rat CTSK (Proteintech, 11239‐1‐AP, 1:200), TNF‐α (Proteintech, 60291‐1‐Ig, 1:200) and IL‐1β (Proteintech, 26048‐1‐AP, 1:200) were used.

Techniques: Immunohistochemical staining, Staining

Journal: Bioactive Materials

Article Title: Bioelectric signals promote diabetic bone regeneration through Piezo1-mediated activation of the efferocytic immune microenvironment

doi: 10.1016/j.bioactmat.2026.05.037

Figure Lengend Snippet: Schematic illustration of Gel BC@ZnO synthesis and the proposed bioelectric signal-Piezo1-Ca 2+ -efferocytosis mechanism for diabetic bone repair.

Article Snippet: Cells were permeabilized with 0.5% Triton X-100, blocked with 5% BSA, and incubated overnight at 4 °C with anti-Piezo1 primary antibody (Abclonal; 1:100) in immunostaining dilution buffer (Beyotime Biotechnology, China).

Techniques:

Journal: Bioactive Materials

Article Title: Bioelectric signals promote diabetic bone regeneration through Piezo1-mediated activation of the efferocytic immune microenvironment

doi: 10.1016/j.bioactmat.2026.05.037

Figure Lengend Snippet: Ultrasound-triggered Gel BC@ZnO promotes Piezo1 activation and macrophage efferocytosis. A) Piezo1 immunofluorescence staining. B) Representative flow-cytometry plots showing CFSE signal in gated macrophages after co-culture with CFSE-labeled apoptotic Jurkat cells. Before co-culture, camptothecin-induced Jurkat cells were quality-checked by Annexin V/7-AAD staining to ensure an apoptosis-enriched target-cell population and minimize necrotic/debris contamination. C) Fluorescence images showing intracellular CFSE signals in macrophages. D) Quantification of Piezo1 immunofluorescence. E) Quantification of CFSE-positive macrophages by flow cytometry. F) Percentage of efferocytic macrophages, defined as F4/80+CFSE + events among total F4/80+ macrophages after exclusion of debris, doublets, and F4/80-negative free apoptotic cells/debris. G) Quantification of intracellular CFSE fluorescence intensity. Data are presented as mean ± SD; error bars indicate SD. Statistical comparisons were performed using one-way ANOVA followed by Tukey's multiple-comparison test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, n = 3.

Article Snippet: Cells were permeabilized with 0.5% Triton X-100, blocked with 5% BSA, and incubated overnight at 4 °C with anti-Piezo1 primary antibody (Abclonal; 1:100) in immunostaining dilution buffer (Beyotime Biotechnology, China).

Techniques: Activation Assay, Immunofluorescence, Staining, Flow Cytometry, Co-Culture Assay, Labeling, Fluorescence, Comparison

Journal: Bioactive Materials

Article Title: Bioelectric signals promote diabetic bone regeneration through Piezo1-mediated activation of the efferocytic immune microenvironment

doi: 10.1016/j.bioactmat.2026.05.037

Figure Lengend Snippet: Gel BC@ZnO + US induces Piezo1-dependent Ca 2+ influx and macrophage immunomodulation in vitro. A, B) Representative flow-cytometry plots and quantification of FITC-Ca 2+ -positive macrophages. C, D) Representative fluorescence images and MFI quantification of intracellular Ca 2+ in macrophages. E, F) Representative flow-cytometry plots and quantification of FITC-Ca 2+ -positive osteoblastic cells. G, H) Representative fluorescence images and MFI quantification of intracellular Ca 2+ in osteoblastic cells. I) Flow-cytometry analysis of macrophage polarization based on CD206 and CD86 expression. J, K) Representative flow-cytometry histograms and quantification of intracellular ROS. GsMTx4 was used as a Piezo1 inhibitor. Data are presented as mean ± SD; error bars indicate SD. Statistical significance was determined by one-way ANOVA with Tukey's multiple-comparison test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, n = 3. Scale bars: 100 μm in C and 150 μm in G.

Article Snippet: Cells were permeabilized with 0.5% Triton X-100, blocked with 5% BSA, and incubated overnight at 4 °C with anti-Piezo1 primary antibody (Abclonal; 1:100) in immunostaining dilution buffer (Beyotime Biotechnology, China).

Techniques: In Vitro, Flow Cytometry, Fluorescence, Expressing, Comparison

Journal: Bioactive Materials

Article Title: Bioelectric signals promote diabetic bone regeneration through Piezo1-mediated activation of the efferocytic immune microenvironment

doi: 10.1016/j.bioactmat.2026.05.037

Figure Lengend Snippet: Gel BC@ZnO + US accelerates diabetic bone-defect repair in vivo. A) Micro-CT images showing new bone formation in diabetic rat femoral condyle defects 8 weeks after surgery. B) Quantitative micro-CT analysis of regenerated bone. C) H&E and Masson's trichrome staining after 8 weeks. D) Piezo1 immunofluorescence staining in the defect area. E) Semi-quantitative analysis of Piezo1-positive area. Data are presented as mean ± SD; error bars indicate SD. Statistical comparisons were performed using one-way ANOVA followed by Tukey's multiple-comparison test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, n = 3.

Article Snippet: Cells were permeabilized with 0.5% Triton X-100, blocked with 5% BSA, and incubated overnight at 4 °C with anti-Piezo1 primary antibody (Abclonal; 1:100) in immunostaining dilution buffer (Beyotime Biotechnology, China).

Techniques: In Vivo, Micro-CT, Staining, Immunofluorescence, Comparison